Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection

Authors

  • Sri Masyeni Department of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Warmadewa, Bali, Indonesia; Department of Internal Medicine, Sanjiwani Hospital, Bali, Indonesia
  • Kuntaman Kuntaman Department of Medical Microbiology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia; Department of Medical Microbiology, Dr. Soetomo Hospital, Surabaya, Indonesia
  • Aryati Aryati Clinical Pathology Department, School of Medicine and Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
  • Muchlis AU Sofro Department of Internal Medicine, Faculty of Medicine, Diponegoro University, Semarang, Indonesia; Department of Internal Medicine, Dr Kariadi Hospital, Semarang, Indonesia
  • Usman Hadi Department of Internal Medicine, Faculty of Medicine, Airlangga University, Surabaya, Indonesia; Department of Internal Medicine, Dr. Soetomo Hospital, Surabaya, Indonesia
  • Gondo Mastutik Department of Anatomical Pathologic, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
  • Windu Purnomo Department of Biostatistics, Faculty of Public Health, Universitas Airlangga Surabaya, Indonesia
  • Agus Santosa Departement of Internal Medicine, Faculty of Medicine and Health Sciences, Universitas Warmadewa, Bali, Indonesia
  • Benediktus Yohan Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Erni Juwita Nelwan Division of Tropical and Infectious Disease, Department of Internal Medicine, Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia; Infectious Disease and Immunology Research Center, Indonesia Medical and Education Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • R. Tedjo Sasmono Eijkman Institute for Molecular Biology, Jakarta, Indonesia

DOI:

https://doi.org/10.52225/narraj.v1i1.31

Keywords:

dengue, microRNA, miR-150, hsa-let-7e, miR-146a

Abstract

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.

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Published

2021-04-01

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Original Article

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