Narra J https://narraj.org/main <div id="groups"> <div id="groups"> <div class="group"> <h2>Editor in Chief</h2> <div class="editorial-list"> <ul class="list-unstyled"> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/harapan.jpg" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Harapan Harapan, MD., PhD</strong></li> <li>Medical Research Unit, School of Medicine</li> <li>Universitas Syiah Kuala, Indonesia</li> <li>Scopus ID : <a title="Scopus ID" href="https://www.scopus.com/authid/detail.uri?authorId=55844857500" target="_blank" rel="noopener" data-toggle="tooltip">55844857500</a></li> </ul> </div> </div> </li> </ul> </div> </div> <div class="group"> <h2>Co-Editor in Chief</h2> <div class="editorial-list"> <ul class="list-unstyled"> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/kuldeep-dhama.jpg" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Kuldeep Dhama, MVSc., PhD</strong></li> <li>NAAS Associate, Principal Scientist</li> <li>ICAR-Indian Veterinary Research Institute, India</li> <li>Scopus ID : <a title="Scopus ID" href="https://www.scopus.com/authid/detail.uri?authorId=6507396956" target="_blank" rel="noopener" data-toggle="tooltip">6507396956</a></li> <li>Email : <a href="https://narraj.org/main/management/settings/context#masthead/kdhama@rediffmail.com">kdhama@rediffmail.com</a></li> </ul> </div> </div> </li> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/abram-l-wagner.jpg" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Abram L. Wagner, PhD, MPH</strong></li> <li>School of Public Health</li> <li>University of Michigan</li> <li>Scopus ID : <a title="Scopus ID" href="https://www.scopus.com/authid/detail.uri?authorId=56178049300" target="_blank" rel="noopener" data-toggle="tooltip">56178049300</a></li> <li>Email : <a href="https://narraj.org/main/management/settings/context#masthead/awag@umich.edu">awag@umich.edu</a></li> </ul> </div> </div> </li> </ul> </div> </div> <div class="group"> <h2>Editorial Board</h2> <div class="editorial-list"> <ul class="list-unstyled"> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/ruth-müller.png" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Ruth Müller, PhD</strong></li> <li>Department of Biomedical Sciences</li> <li>Institute of Tropical Medicine, Belgium</li> <li>Scopus ID : <a title="Scopus ID" href="https://www.scopus.com/authid/detail.uri?authorId=25653255200" target="_blank" rel="noopener" data-toggle="tooltip">25653255200</a></li> <li>Email : <a href="https://narraj.org/main/management/settings/context#masthead/mailto:rmuller@itg.be">rmuller@itg.be</a></li> </ul> </div> </div> </li> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/mahir-gachabayov.jpeg" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Mahir Gachabayov, MD., PhD</strong></li> <li>School of Medicine</li> <li>New York Medical College, USA</li> <li>Scopus ID : <a title="Scopus ID" href="https://www.scopus.com/authid/detail.uri?authorId=56626010400" target="_blank" rel="noopener" data-toggle="tooltip">56626010400</a></li> <li>Email : <a href="https://narraj.org/main/management/settings/context#masthead/gachabayovmahir@gmail.com">gachabayovmahir@gmail.com</a></li> </ul> </div> </div> </li> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/morteza-arab.jpg" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Morteza Arab-Zozani, PhD</strong></li> <li>School of Public Health</li> <li>Birjand University of Medical Sciences (BUMS), Iran</li> <li>Scopus ID : <a title="Scopus ID" href="https://www.scopus.com/authid/detail.uri?authorId=57210284593" target="_blank" rel="noopener" data-toggle="tooltip">57210284593</a></li> <li>Email : <a href="https://narraj.org/main/management/settings/context#masthead/arab.hta@gmail.com">arab.hta@gmail.com</a></li> </ul> </div> </div> </li> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/talha-bin-emran.jpg" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Talha Bin Emran, PhD</strong></li> <li>Department of Pharmacy</li> <li>BGC Trust University Bangladesh, Bangladesh</li> <li>Scopus ID : <a title="Scopus ID" href="https://www.scopus.com/authid/detail.uri?authorId=55325267100" target="_blank" rel="noopener" data-toggle="tooltip">55325267100</a></li> <li>Email : <a href="https://narraj.org/main/management/settings/context#masthead/talhabmb@bgctub.ac.bd">talhabmb@bgctub.ac.bd</a></li> </ul> </div> </div> </li> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/dina-nur-anggraini-ningrum.png" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Dina Nur Anggraini Ningrum, PhD</strong></li> <li>Department of Public Health</li> <li>Universitas Negeri Semarang, Indonesia</li> <li>Scopus ID : <a title="Scopus ID" href="https://www.scopus.com/authid/detail.uri?authorId=57195329470" target="_blank" rel="noopener" data-toggle="tooltip">57195329470</a></li> <li>Email : <a href="https://narraj.org/main/management/settings/context#masthead/dinanan@mail.unnes.ac.id">dinanan@mail.unnes.ac.id</a></li> </ul> </div> </div> </li> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/arief-budi-witarto.png" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Arief Budi Witarto, PhD</strong></li> <li>Department of Biochemistry and Molecular Biology</li> <li>Indonesian Defense University, Indonesia</li> <li>Scopus ID : <a title="Scopus ID" href="https://www.scopus.com/authid/detail.uri?authorId=6507004235" target="_blank" rel="noopener" data-toggle="tooltip">6507004235</a></li> <li>Email : <a href="https://narraj.org/main/management/settings/context#masthead/arief.witarto@idu.ac.id">arief.witarto@idu.ac.id</a></li> </ul> </div> </div> </li> </ul> </div> </div> <div class="group"> <h2>Managing Editor</h2> <div class="editorial-list"> <ul class="list-unstyled"> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/henry-surendra.png" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Henry Surendra, PhD</strong></li> <li>Eijkman-Oxford Clinical Research Unit, Indonesia</li> </ul> </div> </div> </li> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/muhammad-iqhrammullah.jpg" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Muhammad Iqhrammullah, M.S</strong></li> <li>Universitas Syiah Kuala, Indonesia</li> </ul> </div> </div> </li> <li> <div class="img-profile"> <div class="imgthumb"><img src="https://narraj.org/public/site/images/narradmin/baidillah-zulkifli.png" alt="Profile Image" width="75" height="100" /></div> </div> <div class="team-content"> <div class="team-aff"> <ul class="list-unstyled"> <li><strong>Baidillah Zulkifli, M.VetMed</strong></li> <li>Universitas Syiah Kuala, Indonesia</li> </ul> </div> </div> </li> </ul> </div> </div> </div> </div> Narra Sains Indonesia en-US Narra J 2807-2618 Diagnostic performance of GeneXpert MTB/RIF assay compared to conventional Mycobacterium tuberculosis culture for diagnosis of pulmonary and extrapulmonary tuberculosis, Nepal https://narraj.org/main/article/view/33 <div> <div> <p class="NAbstractNEW"><span lang="EN-US">Tuberculosis is an infectious disease caused by the <em>Mycobacterium tuberculosis</em>. It is a global health problem and major cause of death in resource-limited countries like Nepal. Timely diagnosis with sensitive testing methods could assist in early management of the disease. This study was conducted to compare the diagnostic performance of GeneXpert MTB/RIF and conventional acid-fast staining with <em>M. tuberculosis</em> culture. The study was carried out in the Department of Microbiology, Shree Birendra Army Hospital, Nepal. Samples (n=500) were tested with a GeneXpert MTB/RIF assay and acid-fast bacilli (AFB) smear microscopy. All samples were sent for <em>M. tuberculosis</em> conventional culture by the German-Nepal Tuberculosis Project, Kathmandu, Nepal (GENETUP). Out of a total 500 pulmonary and extrapulmonary samples tested, 97 samples were positive for <em>M. tuberculosis</em> by GeneXpert MTB/RIF assay. Out of the positive samples, only 95 samples were found positive by the culture method. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of AFB microscopy was 45.3%, 99.5%, 99.5% and 88.5%, respectively. The sensitivity, specificity, PPV and NPV of GeneXpert MTB/RIF was found to be 100%, 99.5%, 97.5% and 100%, respectively compared to the gold standard culture method. The GeneXpert MTB/RIF test was comparable with culture diagnosis of both pulmonary and extrapulmonary tuberculosis cases. </span></p> </div> </div> Raina Chaudhary Sabita Bhatta Alina Singh Manoj Pradhan Brijendra Shrivastava Yengkokpam I. Singh Ranjit Sah Zareena Fathah Rachana Mehta Ali A. Rabaan Alfonso J. Rodriguez-Morales Kuldeep Dhama Copyright (c) 2021 Narra J 2021-08-01 2021-08-01 1 2 10.52225/narraj.v1i2.33 Impact of economic disruptions and disease experiences on COVID-19 vaccination uptake in Asia: A study in Malaysia https://narraj.org/main/article/view/42 <div> <div> <p class="NAbstractNEW"><span lang="EN-US">During the coronavirus disease 2019 (COVID-19) pandemic, individuals have had a variety of experiences with the disease and economic disruptions in Asia. We assessed how these experiences could impact COVID-19 vaccination intent and uptake in one of the Asian country, Malaysia. Two opt-in internet-based cross-sectional samples were collected: a March wave (26 March – 7 April 2021) and a June wave (22 June – 10 July 2021). Individuals were asked about their vaccination status, their employment status, and their experience with COVID-19 cases. The impact of economic disruptions and experiences with COVID-19 on COVID-19 vaccination was assessed through a multivariable, multinomial logistic regression model. Among 1,493 participants (735 in March and 758 in June wave), 26% were already vaccinated, 57% planned to vaccinate, and 17% had no plan to vaccinate. The number who had lost a job or earned less because of the pandemic was 30% in March and 36% in June. Across both waves, 5%-6% had a personal, very serious experience of COVID-19, 13%-16% knew of a family member or friend with a very serious experience of COVID-19, and 43%-61% knew of a very serious COVID-19 case through media. Notably, compared to those who worked the same amount throughout the pandemic, those who lost their job had lower odds of already being vaccinated (OR: 0.37; 95%CI: 0.23, 0.59), but similar odds of planning to become vaccinated. Personal, family/friend, and media experiences were also all related to increased odds of planning to vaccinated or being already vaccinated. The COVID-19 pandemic has led to large disruptions in people’s lives. People’s experiences during the pandemic impact their likelihood of being vaccinated or planning to vaccinate against COVID-19. Equitable allocation of COVID-19 vaccines will require outreach to groups with less stable employment and can leverage people’s experiences with disease during the pandemic.</span></p> </div> </div> Abram L. Wagner Yogambigai Rajamoorthy Niazlin M. Taib Copyright (c) 2021 Narra J 2021-08-01 2021-08-01 1 2 10.52225/narraj.v1i2.42 Molecular docking of two cytotoxic compounds from Calotropis gigantea leaves against therapeutic molecular target of pancreatic cancer https://narraj.org/main/article/view/37 <div> <div> <p class="NAbstractNEW"><span lang="EN-US">The utilization of natural compounds as therapeutic agents to treat pancreatic cancer has recently focused on natural drug research. <em>Calotropis gigantea</em> has long been believed to be a medicinal plant that helps in treating various diseases. The bioactive compounds 9-metoxipinoresinol and isoliquiritigenin isolated from <em>C. gigantea</em> leaves are proven to act as therapeutic agents by inhibiting the cancer cell growth of Panc-1 cells. This study aimed to screen the potential molecular inhibition mechanisms of 9-metoxipinoresinol and isoliquiritigenin against pancreatic cancer development <em>in-silico</em>. We analyzed the activity of the aforementioned two compounds as inhibitors of several proteins that play a role in the growth of pancreatic cancer cells, such as GCNT3, GOT1, c-Met, PPARγ, BUB1, and NF-κβ, through molecular docking investigation. Our data suggested that 9-metoxipinoresinol and isoliquiritigenin were able to have well interaction with the target proteins, in which the predicted affinity energy ranged between -6.8 and 8.7 kcal/mol. The docking scores of 9-metoxipinoresinol and isoliquiritigenin were higher than the standard drug used (gemcitabine). Based on the binding affinity energy, GCNT3 and BUB1 are potentially to be used as target molecules for cancer therapy using 9-metoxipinoresinol and isoliquiritigenin, respectively. </span></p> </div> </div> Agnia Purnama Vivi Mardina Kana Puspita Intan Qanita Diva R. Rizki Kartini Hasballah Mudassar Iqbal Murniana Sarong Copyright (c) 2021 Narra J 2021-08-01 2021-08-01 1 2 10.52225/narraj.v1i2.37 Peroxidative degradation of proteins and lipids in undifferentiated connective tissue dysplasia in children, Ukraine https://narraj.org/main/article/view/41 <div> <div> <p class="NAbstractNEW"><span lang="EN-US">The aim of the study was to assess the state of lipid and protein peroxidation in children with undifferentiated connective tissue dysplasia (UCTD). In this study, 63 children (33 children with UCTD and 30 children without UCTD were recruited and the indicators of lipid and protein peroxidation were measured. The enzymatic, colorimetric method was used to measure the level of total cholesterol (TC). The phospholipids were analyzed by liquid chromatography/mass spectrometry using electrospray ionization. Lipid peroxidation was studied by assessing the change of index and the end product of lipid peroxidation, malondialdehyde (MDA), using spectrophotometric method. Protein peroxidation - by the content of carbonylated protein based on 2,4 dinitrophenyl-hydrazones derivatization. The activity of the antioxidant defense system enzymes was assessed by measuring catalase (CAT) and superoxide dismutase (SOD). Our study revealed a significant increase of PPO products in the venous blood plasma and LPO products in erythrocytes in children with UCTD. Furthermore, an imbalance of the antioxidant defense system was observed in both blood plasma and erythrocytes membrane. </span></p> </div> </div> Tetyana V. Pochynok Maryna M. Vasiukova Iryna S. Kudlatska-Tyshko Copyright (c) 2021 Narra J 2021-08-01 2021-08-01 1 2 10.52225/narraj.v1i2.41 Persistence of long COVID symptoms in COVID-19 survivors worldwide and its potential pathogenesis - A systematic review and meta-analysis https://narraj.org/main/article/view/36 <div><span lang="EN-US">The study sought to determine the prevalence of persistent long COVID symptoms such as anxiety, depression, dizziness, chest pain, sleep difficulty, palpitations, weight loss, and hair loss among coronavirus disease 2019 (COVID-19) survivors worldwide and to discuss the potential pathogeneses. Potential studies were searched in three databases (PubMed, Scopus, and Web of Science) as of January 30, 2021. Data on study characteristics, patient characteristics during the follow-up, the number of patients with persistent long COVID symptoms and total COVID-19 survivors were collected according to PRISMA guidelines. To assess the quality of studies, the Newcastle-Ottawa scale was used. The estimated prevalence of each long COVID symptom and the association between COVID-19 severity and the occurrence of prolonged symptoms was assessed, if appropriate. The global prevalence of prolonged anxiety was 15.76% (95%CI: 6.36%, 25.15%). Chest pain persisted in 10.36% (239/3,224) of COVID-19 patients (95%CI: 4.92%, 15.80%). Prolonged depression was found in 24 of 548 COVID-19 survivors with an estimated prevalence of 4.32% (95%CI: 2.62%, 6.03%) and dizziness was presented in 4.83% (118/2,219, 95%CI: 1.50%, 8.16%) after recovery. Hair loss was complained by 527 of 2,251 recovered patients (cumulative prevalence of 24.76%, 95%CI: 19.60%, 29.91%), while weight loss was identified in 37 cases among 452 COVID-19 survivors (8.19%, 95%CI: 5.66%, 10.71%). Prolonged palpitation was experienced by 19.38% (211/1,926) survivors with 95%CI: 2.40%, 41.16%. Sleep difficulty was found in 541 of 2,622 COVID-19 survivors (17.87%, 95%CI: 7.55%, 28.20%). The association between COVID-19 severity and the occurrence of persistent long COVID symptoms was not analyzed due to the lack of data. In conclusion, persistent psychological symptoms are frequently reported among COVID-19 survivors. Follow-up studies with a longer duration and larger population are warranted to assess the extent of prolonged symptoms and the quality of life of COVID-19 survivors. Despite various potential pathogeneses that have been hypothesized, a definitive mechanism is yet to be addressed. </span></div> Marhami Fahriani Muhammad Ilmawan Jonny K. Fajar Helnida A. Maliga Andri Frediansyah Sri Masyeni Hanifah Yusuf Firzan Nainu Francesco Rosiello Salin Sirinam Synat Keam Youdiil Ophinni Copyright (c) 2021 Narra J 2021-08-01 2021-08-01 1 2 10.52225/narraj.v1i2.36 SARS-CoV-2 and Orientia tsutsugamushi co-infection in a young teen, Nepal: Significant burden in limited-resource countries in Asia? https://narraj.org/main/article/view/34 <div> <div> <p class="NAbstractNEW"><span lang="EN-US">Scrub typhus is caused by <em>Orientia tsutsugamushi</em>, transmitted through bites of infected chiggers (larval mites). During the coronavirus disease 2019 (COVID-19) pandemic, reports of co-infections with endemic pathogens are increasing around the world. Disease with similar clinical presentation may mask other disease diagnosis and increase the morbidity and mortality of the patients. We report co-infection between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and <em>O. tsutsugamushi</em> in a patient in Nepal presenting with fever, headache, retro-orbital pain, generalized body ache, and knee joints pain with a history of dry cough and dyspnea at night. Since scrub typhus is prevalent and considerate as one of the public health consents in Asian countries and the possible overlapping clinical manifestation with other infections including COVID-19, a further investigation required to determine the burden of SARS-CoV-2 and <em>O. tsutsugamushi</em> co-infection in scrub typhus-endemic countries in Asia.</span></p> </div> </div> Anup Bastola Ranjit Sah Sagar K. Rajbhandari Runa Jha Zareena Fathah Bimal S. Chalise Bikesh Shrestha Rajesh K. Shah Pujan Balla Richa Nepal Bipin Adhikari Ali A. Rabaan Kuldeep Dhama Alfonso J. Rodriguez-Morales Copyright (c) 2021 Narra J 2021-08-01 2021-08-01 1 2 10.52225/narraj.v1i2.34